Compare source conditions and connect them to a measured preparation plan.
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Compare 29 cell-line references
Compare the supplier product, medium and atmosphere in one place, then load the relevant cell line into your workspace. Matching a cell-line name alone does not establish that its culture history or formulation matches yours.
What changes between your cell lines?
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Identity and stock: match the catalogue and derivative to your cells.
Medium and atmosphere: keep the complete formulation and gas setting together.
Calculation basis: distinguish cells/cm², cells/mL and cells per well; these are different inputs.
Uncertainty: an unverified parameter needs your own confirmed input, not a value borrowed from another line.
For worked interpretations, open the expanded HeLa, K-562, MDA-MB-231 and MCF7 pages. Other records remain concise supplier references in this directory.
Growth type
Showing 29 of 29 references
A549
ATCC CCL-185
Human · Lung
Adherent
F-12K 10% FBS · 37°C · 5% CO₂
Passaging, freezing and source limits
Passaging: ATCC gives a reference split range of 1:3–1:8 and medium renewal 2–3 times per week.
Cryopreservation: ATCC lists complete growth medium with 5% v/v DMSO.
Population doubling: 22 hours in the source observation; not a forecast for your stock.
The approximately 22-hour doubling time is an ATCC reference observation, not a prediction for your culture.
Passaging: ATCC reference: Subculture by 80% confluence, seeding 9,000–20,000 viable cells/cm²; renew medium every 2–3 days.
Cryopreservation: ATCC lists complete growth medium with 5% v/v DMSO.
Population doubling: No verified value stored; use a measurement from your culture.
This record covers myoblast growth, not differentiation. ATCC cautions that allowing cultures to become confluent can deplete the myoblastic population.
Passaging: ATCC reference: 1:3–1:6, or 30,000–200,000 viable cells/cm² for subculture; renew medium twice weekly. Retain floating cells during passaging and follow the full product procedure.
Cryopreservation: ATCC lists complete growth medium with 5% v/v DMSO.
Population doubling: 34 hours in the source observation; not a forecast for your stock.
This record is LNCaP clone FGC. Cells attach loosely and can grow in clusters without a uniform confluent monolayer. ATCC advises leaving cultures undisturbed for the first 48 hours after thawing or subculture. The approximately 34-hour doubling time is a reference observation, not a forecast for your culture.
Passaging: ATCC gives a reference split range of 1:3–1:6, or 4 × 10⁴–1 × 10⁵ viable cells/cm².
Cryopreservation: ATCC lists complete growth medium with 5% v/v DMSO.
Population doubling: No verified value stored; use a measurement from your culture.
Human recombinant insulin: The current ATCC entry lists 1.4 mL of a 4 mg/mL insulin stock without an explicit final batch volume in that instruction. A scalable target concentration has not been verified. Enter the target from your lab protocol.
Media calculation needs your lab-confirmed insulin target. Remaining handling fields are not yet verified.
Passaging: ATCC reference: 1:2–1:5, or 10,000–60,000 viable cells/cm²; renew medium 1–2 times weekly.
Cryopreservation: ATCC lists complete growth medium with 5% v/v DMSO.
Population doubling: No verified value stored; use a measurement from your culture.
Finite-lifespan lung fibroblasts. The vendor example specifies non-heat-inactivated FBS and cautions against discarding floating cells without checking viability.
Passaging: ATCC reference: seed 3,000–5,000 cells/cm². Subculture at least twice weekly at 80% confluence or less; renew medium twice weekly.
Cryopreservation: ATCC lists complete growth medium with 5% v/v DMSO.
Population doubling: No verified value stored; use a measurement from your culture.
This is the multiclonal NIH/3T3 line, not NIH/3T3.2 or 3T3-L1. ATCC no longer tests CRL-1658 for contact inhibition because that characteristic has diminished. The current product record specifies 10% FBS; match the serum type to your product and lab protocol.
Passaging: ATCC reference: 1:2–1:8; renew medium 1–2 times weekly.
Cryopreservation: ATCC lists complete growth medium with 5% v/v DMSO.
Population doubling: No verified value stored; use a measurement from your culture.
The referenced L-15 formulation uses atmospheric air without added CO₂. These conditions are not interchangeable with a bicarbonate-buffered medium in 5% CO₂.
Passaging: ATCC reference: 1:2–1:5; renew medium 2–3 times weekly.
Cryopreservation: ATCC lists 95% culture medium and 5% DMSO.
Population doubling: No verified value stored; use a measurement from your culture.
This profile refers specifically to ATCC HTB-14. ATCC identifies it as male in origin and likely a glioblastoma of CNS origin; retain that qualification when describing the line.