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NIH/3T3: reference & planning

Compare source conditions and connect them to a measured preparation plan.

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Cell-line reference

NIH/3T3 CRL-1658

Sourced reference

Vendor conditions describe the referenced product and formulation. Match these to your lab’s approved protocol and culture history.

View source · ATCC

Reviewed 2026-10-08

This is the multiclonal NIH/3T3 line, not NIH/3T3.2 or 3T3-L1. ATCC no longer tests CRL-1658 for contact inhibition because that characteristic has diminished. The current product record specifies 10% FBS; match the serum type to your product and lab protocol.

Culture environment

37°C · 5% CO₂ in air
Adherent · Mouse · Embryo

Medium

ATCC-formulated DMEM
10% FBS (final v/v)

Passaging

ATCC reference: seed 3,000–5,000 cells/cm². Subculture at least twice weekly at 80% confluence or less; renew medium twice weekly.

Trypsin–EDTA; follow the product-specific ATCC procedure.

Growth

Reference data not yet verified.

Cryopreservation

ATCC lists complete growth medium with 5% v/v DMSO.

View source · ATCC

Reviewed 2026-10-08

Use this reference in a calculation

This entry is part of the comparison directory. Compare the supplier formulation and growth type before loading it into a calculator. The linked guides explain how to turn a measured stock and a lab-selected target into a volume plan.

Understand a cell count Interpret a split ratio