Cell Culture
LAB COMPANION
YOUR BENCH WORKSPACE

Cell culture calculators

Select your inputs. Get your quantities.

No account required

Make media

A complete dispensing recipe.

Passage cells

Plan your next subculture.

Seed cells

The right cells in every well.

Freeze cells

Plan your cryovial batch.

Thaw cells

Cell-specific source guidance.

Convert vessels

Scale by culture area.

Media formulation

K-562 · CCL-243
Adjust volume500 mL
1 mL1 mL steps1,000 mL
Batch units

Optional lab choice; not an ATCC default. Confirm your stock strength.

Result units
Dispensing recipeVolume balanced
IMDM base medium
450mL

90% of final volume

Fetal bovine serum
50mL

10% of final volume

Final volume, including all additives500 mL
Calculated from your inputs
How this was calculated

Component volume (mL) = final volume (mL) × percentage ÷ 100.

Supplement volume (mL) = target (mg/mL) × final volume (mL) ÷ stock (mg/mL).

Base = 500 − 50 = 450 mL.

Volumes are additive arithmetic estimates. Calculations retain full numeric precision; displayed values use up to five significant digits.

Target batch
500mL
Serum add
50mL
Base medium
450mL