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Passage cells

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Passage cells

K-562 · CCL-243

Prefilled cell counts and targets are calculation examples. Replace them with your measurements and protocol.

1 · Source culture

Source format

Source vessels harvested

Count all source units pooled into the measured harvest. For plates, count individual wells.

Measured stock concentration

cells/mL

Concentration of the pooled harvest before viability correction.

Viability

Use 100% if your measured count is already viable cells/mL.

Total resuspended harvest volume

Total volume pooled from all source units.

75 cm² per source unit · Nunc 156499

K-562 passage reference

ATCC lists a starting density of 1 × 10⁵ viable cells/mL and subculture at 1 × 10⁶ cells/mL; renew medium every 2–3 days.

View ATCC reference

2 · Destination culture

Destination vessel

Number of vessels

Final volume per well/vessel

Combined cell suspension and medium volume.

Per-well volume units

Plan by

Target basis

Target viable cell density

cells/mL

75 cm² per well/vessel · 8–15 mL per well/vessel reference. Nunc 156499; check your exact product.

View source · Thermo Fisher Scientific

Reviewed 2026-09-28

3 · Optional growth estimateEstimate growth & passage timing

Add your doubling time and target to model a growth curve and the next five passage intervals.

Result units

Your dispensing planCount-based plan

Equivalent split per source

1:9Cells per source ÷ cells per destination

Cells per destination

1,000,000Viable cells per vessel

Harvest fraction per destination

11.111%Of the entire pooled suspension

Maximum destinations

9Vessels at this target density

Ratio uses the average harvest from 1 source unit. Different vessel areas can produce different seeding densities at the same ratio.

Unallocated stock suspension after this transfer: 8.8889 mL.

Viable cells available

9,000,000 cells

Cells required

1,000,000 cells

Cell suspension · total

1.1111 mL

Fresh medium · total

8.8889 mL

Per vessel

1.1111 mL cell suspension + 8.8889 mL fresh medium = 10 mL

1,000,000 cells · 100,000 cells/mL

Calculated from your inputs

How this was calculated

Viable concentration = 1,000,000 × 90/100 = 900,000 cells/mL.

Cells required = 1,000,000 per well/vessel × 1 = 1,000,000.

Suspension = 1,000,000 ÷ 900,000 = 1.1111 mL.

Fresh medium = 10 − 1.1111 = 8.8889 mL.

Equivalent split per source = 9,000,000 ÷ 1,000,000 = 1:9.

Each destination uses 11.111% of the entire pooled harvest.

Volumes are additive arithmetic estimates. Calculations retain full numeric precision; displayed values use up to five significant digits.