Cell culture calculations, explained
Plan quantities, understand the assumptions, and check the result.
Make media
A complete dispensing recipe.
Passage cells
Plan your next subculture.
Seed cells
The right cells in every well.
Freeze cells
Plan your cryovial batch.
Thaw cells
Cell-specific source guidance.
Cell line
Your sourced culture reference.
Plan a complete mixture, account for viable cells, and check whether your stock fits the intended volume. Open a calculator for its formulas and worked examples; use the cell-line references to identify the source behind each formulation.
Media formulation
K-562 · CCL-243Optional lab choice; not an ATCC default. Confirm your stock strength.
90% of final volume
10% of final volume
What a 5 µL seeding plan actually means
Compare 384- and 1536-well preparations, calculate a batch allowance and record which volumes your equipment actually dispenses.
Read the walkthrough →Final medium volume versus a full bottle
Check percentage labels, convert stock strength into a volume fraction, and see why adding to 500 mL changes the concentration.
Read the walkthrough →Interactive example + observation worksheetFrom a counter result to a viable seeding stock
Reconcile total and live reports interactively, apply dilution once, and export a worksheet for the report and observed result.
Read the walkthrough →What does a 1:4 split mean after pooling?
Follow two source flasks into six destinations. Compare a per-source split with a fraction of the whole harvest.
Read the walkthrough →Vessel area is not a viable-cell budget
Convert a T-75 to wells, handle fractional equivalents, and check whether the recovered cells can actually supply the chosen layout.
Read the walkthrough →Interactive example + observation worksheetPlan cryovials by cells, volume and allowance
Check the cell budget including allowance, enter an optional recovery measurement and distinguish recovery from viability.
Read the walkthrough →