From numbers to a usable plan
Practical calculation guides
Six practical walkthroughs connect the calculation to the decision at the bench. Three include interactive bench examples and downloadable observation worksheets. Compare alternative interpretations, reproduce the arithmetic and check which measurements your plan still needs.
What a 5 µL seeding plan actually means
Compare 384- and 1536-well preparations, calculate a batch allowance and record which volumes your equipment actually dispenses.
Read the walkthrough →Final medium volume versus a full bottle
Check percentage labels, convert stock strength into a volume fraction, and see why adding to 500 mL changes the concentration.
Read the walkthrough →Interactive example + observation worksheetFrom a counter result to a viable seeding stock
Reconcile total and live reports interactively, apply dilution once, and export a worksheet for the report and observed result.
Read the walkthrough →What does a 1:4 split mean after pooling?
Follow two source flasks into six destinations. Compare a per-source split with a fraction of the whole harvest.
Read the walkthrough →Vessel area is not a viable-cell budget
Convert a T-75 to wells, handle fractional equivalents, and check whether the recovered cells can actually supply the chosen layout.
Read the walkthrough →Interactive example + observation worksheetPlan cryovials by cells, volume and allowance
Check the cell budget including allowance, enter an optional recovery measurement and distinguish recovery from viability.
Read the walkthrough →Start with the right question
- How much of a stock do I need? Use the measured viable concentration for cells, or the stock strength for a supplement.
- What is the final volume? Count all liquid contributions, including the stock itself.
- Does this number describe one well or the whole batch? Keep the per-unit plan separate from reservoir and transfer allowances.
These are original arithmetic demonstrations, not recommended biological conditions. Each calculator also has its own instructions, formulas and worked examples directly below its controls.
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