Cell Culture
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Freeze cells

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Freeze cells

K-562 · CCL-243

Example inputs for a harvested cell pellet. Use a complete freezing medium from your approved protocol.

ATCC lists complete growth medium with 5% v/v DMSO.

View source · ATCC
Reviewed 2026-09-28
Result units

This calculation assumes a harvested cell pellet with negligible residual liquid. It does not calculate mixing of a cell suspension with concentrated cryoprotectant.

Cryovial allocation
Cells required
5,000,000 viable cells
Maximum full vials available
10 vials
Final freezing-medium resuspension
5 mL
Final concentration
1,000,000 cells/mL

Dispense 1 mL per vial. 5,000,000 viable cells remain unallocated.

Calculated from your inputs
How this was calculated

Required viable cells = cells per vial × number of vials.

Final resuspension volume = volume per vial × number of vials.

Final concentration = cells per vial ÷ volume per vial.

Volumes are additive arithmetic estimates. Calculations retain full numeric precision; displayed values use up to five significant digits.