Cell Culture
LAB COMPANION
YOUR BENCH WORKSPACE

Seed cells

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Seed cells

K-562 · CCL-243

Prefilled cell counts and targets are calculation examples. Replace them with your measurements and protocol.

Destination vessel
Per-well volume units
Target basis

75 cm² per well/vessel · 8–15 mL per well/vessel reference. Nunc 156499; check your exact product.

View source · Thermo Fisher Scientific
Reviewed 2026-09-28
Result units
Your dispensing planCount-based plan
Viable cells available
9,000,000 cells
Cells required
1,000,000 cells
Cell suspension · total
1.1111 mL
Fresh medium · total
8.8889 mL
Per vessel

1.1111 mL cell suspension + 8.8889 mL fresh medium = 10 mL

1,000,000 cells · 100,000 cells/mL

Calculated from your inputs
How this was calculated

Viable concentration = 1,000,000 × 90/100 = 900,000 cells/mL.

Cells required = 1,000,000 per well/vessel × 1 = 1,000,000.

Suspension = 1,000,000 ÷ 900,000 = 1.1111 mL.

Fresh medium = 10 − 1.1111 = 8.8889 mL.

Volumes are additive arithmetic estimates. Calculations retain full numeric precision; displayed values use up to five significant digits.