SW480: reference & planning
Compare source conditions and connect them to a measured preparation plan.
Culture formulation
ATCC referenceLeibovitz’s L-15 · 10% FBS · 37°C · Atmospheric air; no added CO₂
This choice updates the calculator and resets the serum to the selected reference. You can then edit the serum and supplements below.
Transition the cells to a CO₂-dependent medium
- Start from a healthy, established culture; recover newly thawed cells using the supplier’s conditions.
- Keep a parallel culture in the original medium while assessing the alternative.
- Use a lab-approved adaptation plan. Compare growth and morphology before progressing, and check relevant function after adaptation.
Gas exchange follows the formulation, not the passage number. L-15 mixed with bicarbonate-buffered medium needs validated pH and gas conditions. A fixed 75/25 → 50/50 → 25/75 schedule does not establish when to seal or loosen caps.
At the selected 5% CO₂ endpoint, use the exact compatible medium and a vessel that permits gas exchange, such as a vented cap, according to your protocol. DMEM products differ in bicarbonate content.
ATCC: adaptation and buffering guidanceVendor conditions describe the referenced product and formulation. Match these to your lab’s approved protocol and culture history.
Culture environment
37°C · Atmospheric air; no added CO₂
Adherent · Human · Colon
Medium
ATCC-formulated Leibovitz’s L-15
10% FBS (final v/v)
Passaging
ATCC reference: 1:2–1:8; renew medium 1–2 times weekly.
Trypsin–EDTA; follow the product-specific ATCC procedure.
Growth
Reference data not yet verified.
Cryopreservation
ATCC lists complete growth medium with 5% v/v DMSO.
Use this reference in a calculation
This entry is part of the comparison directory. Compare the supplier formulation and growth type before loading it into a calculator. The linked guides explain how to turn a measured stock and a lab-selected target into a volume plan.