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HCT 116: reference & planning
Compare source conditions and connect them to a measured preparation plan.
Vendor conditions describe the referenced product and formulation. Match these to your lab’s approved protocol and culture history.
View source · ATCC
Reviewed 2026-10-01
Culture environment
37°C · 5% CO₂ in air
Adherent · Human · Colon
Medium
ATCC-formulated McCoy’s 5A (modified)
10% FBS (final v/v)
Passaging
ATCC reference: 1:3–1:8; renew medium every 2–3 days.
Trypsin–EDTA; follow the product-specific ATCC procedure.
Growth
Reference data not yet verified.
Cryopreservation
ATCC lists complete growth medium with 5% v/v DMSO.
View source · ATCC
Reviewed 2026-10-01
Use this reference in a calculation
This entry is part of the comparison directory. Compare the supplier formulation and growth type before loading it into a calculator. The linked guides explain how to turn a measured stock and a lab-selected target into a volume plan.