A549: reference & planning
Compare source conditions and connect them to a measured preparation plan.
Vendor conditions describe the referenced product and formulation. Match these to your lab’s approved protocol and culture history.
Culture environment
37°C · 5% CO₂ in air
Adherent · Human · Lung
Medium
ATCC-formulated F-12K
10% FBS (final v/v)
Passaging
ATCC gives a reference split range of 1:3–1:8 and medium renewal 2–3 times per week.
Trypsin–EDTA, following the linked ATCC procedure.
Growth
Approximately 22 hours population doubling time in the ATCC reference.
Cryopreservation
ATCC lists complete growth medium with 5% v/v DMSO.
Use this reference in a calculation
This entry is part of the comparison directory. Compare the supplier formulation and growth type before loading it into a calculator. The linked guides explain how to turn a measured stock and a lab-selected target into a volume plan.