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LNCaP (clone FGC): reference & planning

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Cell-line reference

LNCaP (clone FGC) CRL-1740

Sourced reference

Vendor conditions describe the referenced product and formulation. Match these to your lab’s approved protocol and culture history.

View source · ATCC

Reviewed 2026-10-08

This record is LNCaP clone FGC. Cells attach loosely and can grow in clusters without a uniform confluent monolayer. ATCC advises leaving cultures undisturbed for the first 48 hours after thawing or subculture. The approximately 34-hour doubling time is a reference observation, not a forecast for your culture.

Culture environment

37°C · 5% CO₂ in air
Adherent · Human · Prostate / lymph-node metastasis

Medium

ATCC-formulated RPMI-1640
10% FBS (final v/v)

Passaging

ATCC reference: 1:3–1:6, or 30,000–200,000 viable cells/cm² for subculture; renew medium twice weekly. Retain floating cells during passaging and follow the full product procedure.

Trypsin–EDTA; follow the product-specific ATCC procedure.

Growth

Approximately 34 hours population doubling time in the ATCC reference.

Cryopreservation

ATCC lists complete growth medium with 5% v/v DMSO.

View source · ATCC

Reviewed 2026-10-08

Use this reference in a calculation

This entry is part of the comparison directory. Compare the supplier formulation and growth type before loading it into a calculator. The linked guides explain how to turn a measured stock and a lab-selected target into a volume plan.

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