WI-38: reference & planning
Compare source conditions and connect them to a measured preparation plan.
Vendor conditions describe the referenced product and formulation. Match these to your lab’s approved protocol and culture history.
Culture environment
37°C · 5% CO₂ in air
Adherent · Human · Lung
Medium
ATCC-formulated EMEM
10% FBS (final v/v)
Passaging
ATCC reference: 1:2–1:4; renew medium 2–3 times weekly.
Trypsin–EDTA; follow the product-specific ATCC procedure.
Growth
Approximately 24 hours population doubling time in the ATCC reference.
Cryopreservation
ATCC lists complete growth medium with 5% v/v DMSO.
Use this reference in a calculation
This entry is part of the comparison directory. Compare the supplier formulation and growth type before loading it into a calculator. The linked guides explain how to turn a measured stock and a lab-selected target into a volume plan.